Comparison between Indirect Immunofluorescence Assay and Shell Vial Culture for Detection of Mumps Virus from Clinical Samples
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چکیده
منابع مشابه
Comparison of cytomegalovirus antigenemia assay with shell vial-indirect immunofluorescence assay for rapid detection of cytomegalovirus in blood.
The recent article by Mazzulli et al. (4) further demonstrates the increasing interest of virologists and microbiologists in the cytomegalovirus antigenemia (CMV-Ag) assay. Mazzulli and coworkers report that the CMV-Ag assay is more sensitive than the shell vial-indirect immunofluorescence assay (SVAIFA), perhaps implying that the direct antigen detection assay should be considered the method o...
متن کاملDetection of mumps virus in clinical specimens by rapid centrifugation culture and conventional tube cell culture.
Conventional tube cell culture was compared with a 2 day and 5 day spin-amplified shell vial indirect immunofluorescence assay for the detection of mumps virus in swabs from the area of Stensen's duct. The sensitivity and specificity of the shell vial assay were 95.9 and 100%, respectively. The shell vial detected 66.3% of the positive cultures within 2 days of inoculation while the first posit...
متن کاملEvaluation of different continuous cell lines in the isolation of mumps virus by the shell vial method from clinical samples.
AIMS To compare prospectively the efficacy of the Vero, LLC-MK2, MDCK, Hep-2, and MRC-5 cell lines in the isolation of the mumps virus from clinical samples by means of the shell vial method. METHODS During an epidemic outbreak of parotiditis 48 clinical samples (saliva swabs and CSF) were studied. Two vials of the Vero, LLC-MK2, MDCK, MRC-5, and Hep-2 cell lines were inoculated with 0.2 ml o...
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The isolation of arboviruses from patient's low titer sera can be difficult. Here we compared the detection efficiency of Dengue (DEN), Yellow Fever (YF), Saint Louis Encephalitis (SLE), West Nile (WN), Ilheus (ILH), Group C (GC), Oropouche (ORO), Mayaro (MAY) and Venezuela Encephalitis Equine (VEE) viruses using a Modified Shell Vial Culture (MSVC) protocol to a Standard Cell Culture (SCC) pro...
متن کاملComparison of a modified shell vial culture procedure with conventional mouse inoculation for rabies virus isolation.
Rabies is a neurotropic disease that is often lethal. The early diagnosis of rabies infection is important and requires methods that allow for the isolation of the virus from animals and humans. The present study compared a modified shell vial (MSV) procedure using 24-well tissue culture plates with the mouse inoculation test (MIT), which is considered the gold standard for rabies virus isolati...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2003
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.41.11.5186-5187.2003